C3A-CAS9 cell line

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  • C3A-CAS9 cell line C3A-CAS9-产品图
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C3A-CAS9 cell line C3A-CAS9-产品图
C3A-CAS9 Copy Product Info
Catalog#： YC-C093-Cas9-H
Size： 1*10^6
Culture method： 90%DMEM/F12+10% FBS
STR Report
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C3A-CAS9 Copy Product Info
Catalog#： YC-C093-Cas9-H
Size： 1*10^6
Culture method： 90%DMEM/F12+10% FBS

Detailed Product Information

Cell Info

Product category Cas9 Stable Cell Lines Mycoplasma test negative
Cell Morphology Epithelial-like,adherent Culture method 90%DMEM/F12+10% FBS
Passage ratio 1:3~1:4 Species Human
Expression gene Cas9 Resistance gene hygro
Product category
Cas9 Stable Cell Lines
Mycoplasma test
negative
Cell Morphology
Epithelial-like,adherent
Culture method
90%DMEM/F12+10% FBS
Passage ratio
1:3~1:4
Species
Human
Expression gene
Cas9
Resistance gene
hygro
Recombinant Cas9 protein derived from Streptococcus pyogenes is a protein that plays a key role in CRISPR/Cas9 system. It has endonuclease activity; Under the guidance of sgRNA, Cas9 protein can move to the target gene site, cut DNA accurately, and cause DNA double-strand breaks. Ubigene's C3A cell line was constructed on the basis of wild-type C3A cells by lentiviral transduction of the engineered Cas9 protein gene, which can stably express Cas9 protein for a long time. Using this cell, efficient gene knockout, knock-in and point mutation in C3A cells can be achieved by only transfecting sgRNA or co-transfecting sgRNA and donor DNA. It can also be used for the construction of cell knockout library and easily solve the problem of Cas9 transfection difficulty.
STR Profile
Download STR Report
Loci STR Info (Sample Cell)
Sample Cell Line:C3A		 STR Info (Cell bank)
Cell Line: [HepG2/C3A, derivative of Hep G2 (ATCC HB-8065)]
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11131113
D13S317 913913
D7S820 10101010
D16S539 12131213
VWA 17171717
TH01 9999
AMEL XYXY
TPOX 8989
CSF1PO 10111011
D12S391 2125
FGA 2225
D2S1338 1920
D21S11 2931
D18S51 1314
D8S1179 1516
D3S1358 1516
D6S1043 1313
PENTAE 1520
D19S433 15.215.2
PENTAD 913
D1S1656 1112
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

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    Easy to use
    Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
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    High-efficient KO
    These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
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    Curated cells
    Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
Related Resources: Red Cotton Gene-editing System | ATCC | DSMZ | ECAZZ | Cellosaurus | CCTCC | NCBI
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